Long-term patch recordings from adult spinal neurons herald new era of opportunity.
نویسنده
چکیده
Recently, Andreas Husch, Nathan Cramer, and Ronald M. Harris-Warrick achieved a remarkable breakthrough in patch-clamp recordings of ventral horn neurons in the adult spinal cord slice preparation. This landmark study that breaks the "age barrier" is titled "Long-duration perforated patch recordings from spinal interneurons of adult mice" (Husch et al., in press). In it, the authors demonstrate the unprecedented ability to undertake day-long (up to 12 h), and utterly stable perforated patch recordings. A description of the methodology is detailed in their paper. Here, I give a brief overview before providing context to this extraordinary achievement.
منابع مشابه
Innovative Methodology Long-duration perforated patch recordings from spinal interneurons of adult mice
Husch A, Cramer N, Harris-Warrick RM. Long-duration perforated patch recordings from spinal interneurons of adult mice. J Neurophysiol 106: 2783–2789, 2011. First published September 7, 2011; doi:10.1152/jn.00673.2011.—It has been very difficult to record from interneurons in acute slices of the lumbar spinal cord from mice 3 wk of age. The low success rate and short recording times limit in vi...
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ورودعنوان ژورنال:
- Journal of neurophysiology
دوره 106 6 شماره
صفحات -
تاریخ انتشار 2011